Deuterium and mRNA Stabilization
Problems Arising from mRNA’s Inherent Instability
mRNA is inherently unstable. It is susceptible to both thermal and enzymatic degradation. mRNA degradation is a temperature dependent process. Higher temperatures will lead to more degradation. Even at refrigerated temperatures of 4°C, mRNA integrity is only maintained for a short period of time. As a result, -80°C freezers are often required to preserve the integrity of mRNA therapeutics.
This is costly and problematic for mRNA storage and distribution; in remote locations with poor infrastructure, maintaining cold chain transportation can be unfeasible.
What is mRNA and why
is it important?
Messenger RNA (mRNA) is a type of
RNA that is required for protein
It differs from DNA, which stores the genetic information in our bodies, mRNA carries the genetic information that direct cells to produce proteins. In the case of vaccines, the proteins can result in an immune response that helps protect against disease.
What is Deuterium?
Deuterium is an isotope of the hydrogen atom. Deuterium oxide (D2O) is a molecule composed of two atoms of deuterium and one atom of oxygen. D2O is non-toxic and behaves like H²O in the body.
D-Lock™ Stabilizes mRNA
Research led by deutraMed scientists demonstrates that D-Lock™ stabilization technology not only successfully transcribes mRNA molecules but significantly retains mRNA integrity compared to conventional synthesis methods.
D-Lock™ Enhances mRNA Transcription and Translation Efficiency
mRNA synthesis using D-Lock™ technology significantly improves both transcription and translation efficiency compared with conventional in vitro technology. Translation refers to the process by which a protein is synthesized from information contained in an mRNA molecule. Transcription is the process by which the genetic information in a strand of DNA is copied into a new molecule of mRNA.
deutraMed’s mRNA stabilization research program is led by the company’s Chief Scientist, Dr. Pavel Gris, PhD, MRSC, C.Chem., and an Adjunct Professor in University of Waterloo’s Department of Chemistry. The work has been conducted with support from deutraMed’s Scientific Advisory Board.